Microscopy, Imaging & Cell Metabolism

Overview of Services

Microscopy Analysis

The Microscopy Core will provide use of microscopes including an Olympus confocal, one transmission electron microscope, a new high content imager (ImageXPress Nano, MHS 16) and one ultramicrotome. Training will be provided to new users. In some cases, core personnel can be hired to perform all aspects of an experiment; in other cases, users may use the equipment on their own. 

Cellular Imaging and Microscopy

The Cellular and Molecular Imaging (CMI) Core supported by the Integrative Neuroscience COBRE center provides users with state-of-the-art instruments and advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs and small animals. The core includes a Leica SP8 confocal with Lightning capabilities, one Scientifica highly customizable two-photon, and two Thunder imagers with computational clearing capabilities (3D Tissue and Model Organism). Training will be provided to new users. In some cases, core personnel can be hired to perform all aspects of an experiment; in other cases, users may use the equipment on their own. The Cellular and Molecular Imaging Core has been opened in 2016, and it is housed within the Department of Biology under the leadership of Dr. Alexander van der Linden.

Cell Metabolism Analysis

The Cell Metabolism Core allows users to measure mitochondrial and non-mitochondrial oxygen consumption and glycolysis in live cells, whole organisms and tissues on a 24 well format or on a 8 well format. There are two instruments available, the XF24 and the XFp Analyzers. The instruments are capable of analyzing up to 6 conditions at four replicates each. The analyzers is located in Manville Health Sciences Room 16 (Sample Prep Room) The Cell Metabolism Core has been opened on February of 2017, and it is housed within the Department of Pharmacology under the leadership of Dr. Ruben Dagda.

Neuroimaging

The Neuroimaging Core provides users with resources to measure neural activity in human subjects. Direct neuronal activity measurement is provided by our two high-density, high-frequency electroencephalography (EEG) systems, while measument of higher spatial acuity is provided by our magnetic resonance imaing (MRI) scanners and near-infrared spectroscopy (NIRS) systems. Additional equipment is available for experimental or diagnostic measurement of: retinal activity (two ERG systems, including multifocal and visual evoked potentials); behavioral visual function including color blindness, visual acuity, contrast perception; and imaging of retinal structures (AOSLO).

This core was supported, in part, by the Cell Biology COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103554). The Leica SP8 confocal was supported, in part, by the Integrative Neuroscience COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103650). The Scientifica 2-photon and Leica Thunder imagers was supported by Integrative Neuroscience COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103650). The iLab license was provided by the NV INBRE Administrative Supplement, a grant from the NIH National Institute of General Medical Sciences (GM103440-14A1S1).

Location and hours of operation 

Links and Resources

• http://med.unr.edu/cobre/cores
• https://www.unr.edu/neuroscience/research/center/core-facilities/cellular-and-molecular-imaging-core
• https://www.unr.edu/neuroscience/research/center/core-facilities/neuroimaging-core

Contacts