Microscopy, Imaging & Cell Metabolism

Overview of Services

Microscopy Analysis

The Microscopy Core will provide use of microscopes including an Olympus confocal, one transmission electron microscope, a new high content imager (ImageXPress Nano, MHS 16) and one ultramicrotome. Training will be provided to new users. In some cases, core personnel can be hired to perform all aspects of an experiment; in other cases, users may use the equipment on their own. 

Cellular Imaging and Microscopy

The Cellular and Molecular Imaging (CMI) Core supported by the Integrative Neuroscience COBRE center provides users with state-of-the-art instruments and advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs and small animals. The core includes a Leica SP8 confocal with Lightning capabilities, one Scientifica highly customizable two-photon, and two Thunder imagers with computational clearing capabilities (3D Tissue and Model Organism). Training will be provided to new users. In some cases, core personnel can be hired to perform all aspects of an experiment; in other cases, users may use the equipment on their own. The Cellular and Molecular Imaging Core has been opened in 2016, and it is housed within the Department of Biology under the leadership of Dr. Alexander van der Linden.

Cell Metabolism Analysis

The Cell Metabolism Core allows users to measure mitochondrial and non-mitochondrial oxygen consumption and glycolysis in live cells, whole organisms and tissues on a 24 well format or on a 8 well format. There are two instruments available, the XF24 and the XFp Analyzers. The instruments are capable of analyzing up to 6 conditions at four replicates each. The analyzers is located in Manville Health Sciences Room 16 (Sample Prep Room) The Cell Metabolism Core has been opened on February of 2017, and it is housed within the Department of Pharmacology under the leadership of Dr. Ruben Dagda.

Neuroimaging

The Neuroimaging Core provides users with resources to measure neural activity in human subjects. Direct neuronal activity measurement is provided by our two high-density, high-frequency electroencephalography (EEG) systems, while measument of higher spatial acuity is provided by our magnetic resonance imaing (MRI) scanners and near-infrared spectroscopy (NIRS) systems. Additional equipment is available for experimental or diagnostic measurement of: retinal activity (two ERG systems, including multifocal and visual evoked potentials); behavioral visual function including color blindness, visual acuity, contrast perception; and imaging of retinal structures (AOSLO).

 

This core was supported, in part, by the Cell Biology COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103554). The Leica SP8 confocal was supported, in part, by the Integrative Neuroscience COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103650). The Scientifica 2-photon and Leica Thunder imagers was supported by Integrative Neuroscience COBRE, a grant from the NIH, National Institute of General Medical Sciences (GM103650). The iLab license was provided by the NV INBRE Administrative Supplement, a grant from the NIH National Institute of General Medical Sciences (GM103440-14A1S1).

Location and hours of operation 

Hours Open

 

Hours Staffed

 

Location

 

Monday - Friday
8am - 5pm

 

Monday - Friday
8am - 5pm

 

Olympus Confocal
Howard 144
1664 North Virginia
Reno, NV, 89557

 

Monday - Friday
8am - 5pm

 

Monday - Friday
9am - 5pm

 

Transmission Electron Microscope; Ultramicrotome
Manville 14
1664 North Virginia
Reno, NV, 89557

 

Monday - Friday
24/7

 

Monday - Friday
9am - 5pm

 

Leica SP8 Confocal, Thunders, Scientifica 2-Photon 
Fleischmann Life Sciences (324, 318)
1664 North Virginia
Reno, NV, 89557

 

Monday - Friday
7am - 5pm

 

Monday - Friday
9am - 5pm

 

Cell Metabolism Analysis and High Content Imager
Manville Health Sciences Building 16 (Sample Prep Room)
1664 North Virginia
Reno, NV, 89557

 

Monday - Friday
After 6pm by appointment

Saturday - Sunday
By appointment

By appointment

Siemens Skyra MRI scanner
Rewnown Health X-Ray and Imaging, South Meadows
10085 Double R Blvd
Reno, NV, 89521

Monday - Sunday
24/7

Monday - Friday
9am - 5pm

COBRE Core B Neuroimaging Suite
Effie Mona Mack Building Suite 300
1664 North Virginia
Reno, NV, 89557

Links and Resources

Contacts

Name Role Phone Email Location
Chris von Bartheld
Cell Biology of Signaling Across Membranes, COBRE Core Director
 
775-784-6022
 
cvonbartheld@med.unr.edu
 
Manville 3
 
Alexander van der Linden
Cellular and Molecular Imaging (CMI) Core Director, COBRE Integrative Neuroscience
 
775-784-6080
 
avanderlinden@unr.edu
 
Fleischmann Life Sciences, room 339
 
Robert Renden
Oversee use of School of Medicine confocal microscope
 
775-784-4352
 
renden@unr.edu
 
Manville 12A
 
Ruben K. Dagda
Oversee use of Seahorse instruments
 
775-784-4121
 
rdagda@med.unr.edu
 
Manville Health Sciences 8E and 19
 
Raul Dagda
Cell Metabolism Core Assistant/ Co-Director
 
775-784-4769
 
rauld@med.unr.edu
 
Manville Health Sciences 8E
 
Lars Strother
Director, Neuroimaging
 
775-784-8678
 
lars@unr.edu
 
Mack Social Science, room 406
 
Smijin Soman
Manager of the high content ImageXPress Nano
 
775-784-4121
 
skarthullysoman@med.unr.edu
 
Howard Medical Sciences room 148
 
Andrew Yanez
CMI Core Specialist, COBRE Integrative Neuroscience
 

 
ayanez@unr.edu
 
Fleishmann Life Sciences, room 311H