ALERT

In Observance of U.S. holiday, Independence Day, Agilent CrossLab, iLab Operations Software will be closed during U.S. hours and have support during EU and APAC hours on Monday July 3rd and Tuesday July 4th, 2017. We will resume U.S. support hours on Wednesday July 5th, 2017. For urgent matters, please add "Urgent" to the ticket subject and we will prioritize those requests first.

 

Thank You!

Single Cell Molecular Expression

Overview of Services


The Single Cell Molecular Expression Core (SCME) provides the following state of the art services:

 

  • Automated single cell capture followed by cDNA preparation using the Fluidigm C1 Single Cell Auto-Prep system
  • High throughput quantitative PCR assays and targeted template enrichment using the Fluidigm BioMark HD system
  • Manipulation of a gene of interest to achieve over-expression, reduced expression, complete inactivation, or replacement with another gene using the latest genome editing technologies including ZFNs, TALENs and Cas9-CRISPR in cultured cells.
  • Assistance in the designing, execution and analysis of next-generation deep sequencing and genome editing experiments.

 

This core is supported by the Center of Biomedical Research Excellence (COBRE) Smooth Muscle Plasticity Phase III, a grant from the NIH National Institute of General Medical Sciences (Insert Grant Number). The iLab license was provided by the NV INBRE Administrative Supplement, a grant from the NIH National Institute of General Medical Sciences (GM103440-14A1S1).

Leadership

Wei Yan | Director

Chong Tang | Co-Director

Location and hours of operation

Hours Location

Monday - Friday         

9am - 5pm 

Center Molecular Medicine Rm 202 & 204

Reno, NV, 89557 

Links and Resources

Contacts

Name Role Phone Email Location
Wei Yan
Director
 
775-784-7765
 
wyan@medicine.nevada.edu
 
CMM Room L207
 
Chong Tang
Co-Director
 
775-784-4688
 
tangc3@unr.edu
 
CMM Room L204
 
Tian Yu
Assistant Director
 
775-784-6908
 
tiany@nevada.unr.edu
 
CMM Room L204
 
Huili Zheng
Lab Manager
 
775-682-8873
 
hzheng@medicine.nevada.edu
 
CMM Room L203D
 

Services

Name Description
Biomark qPCR service sum 
  • Per sample
 
Bimark HT qPCR service sum 
  • Per sample
 
Fludigm C1 service sum 
  • Per sample
 
NGS RNA library sequencing sum 
  • Per sample
 
NGS DNA library sum 
  • Per sample
 
Small RNA Sequencing sum 
  • Per sample
 
Gene deletion using Cas9/CRISPR in mice 
  • Per gRNA
 
CRISPR in vitro transcription for RNA Injections 
  • Per gRNA
 
Homologous Donor Design and Construction 
  • Per sample
 
Pronuclear Injection (100 embryos/4 surrogates) and Embryo transfer   
Genotyping Design and Service 
  • Per sample
 
Bioinformatics and BioMark Service 
  • Per hour
 
Cage/per day 
  • Price will vary for external/corporate users based off of your project needs.
 

Service list


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Core Services (13)