Single Cell Molecular Expression

Overview of Services


The Single Cell Molecular Expression Core (SCME) provides the following state of the art services:

 

  • Automated single cell capture followed by cDNA preparation using the Fluidigm C1 Single Cell Auto-Prep system
  • High throughput quantitative PCR assays and targeted template enrichment using the Fluidigm BioMark HD system
  • Manipulation of a gene of interest to achieve over-expression, reduced expression, complete inactivation, or replacement with another gene using the latest genome editing technologies including ZFNs, TALENs and Cas9-CRISPR in cultured cells.
  • Assistance in the designing, execution and analysis of next-generation deep sequencing and genome editing experiments.

 

This core is supported by the Center of Biomedical Research Excellence (COBRE) Smooth Muscle Plasticity Phase III, a grant from the NIH National Institute of General Medical Sciences (Insert Grant Number). The iLab license was provided by the NV INBRE Administrative Supplement, a grant from the NIH National Institute of General Medical Sciences (GM103440-14A1S1).

Leadership

Wei Yan | Director

Chong Tang | Co-Director

Location and hours of operation

Hours Location

Monday - Friday         

9am - 5pm 

Center Molecular Medicine Rm 202 & 204

Reno, NV, 89557 

Links and Resources

Contacts

Name Role Phone Email Location
Wei Yan
Director
 
775-784-7765
 
wyan@medicine.nevada.edu
 
CMM Room L207
 
Chong Tang
Co-Director
 
775-784-4688
 
tangc3@unr.edu
 
CMM Room L204
 
Tian Yu
Assistant Director
 
775-784-6908
 
tiany@nevada.unr.edu
 
CMM Room L204
 
Huili Zheng
Lab Manager
 
775-682-8873
 
hzheng@medicine.nevada.edu
 
CMM Room L203D
 

Services

Name Description
Biomark qPCR service sum 
  • Per sample
 
Bimark HT qPCR service sum 
  • Per sample
 
Fludigm C1 service sum 
  • Per sample
 
NGS RNA library sequencing sum 
  • Per sample
 
NGS DNA library sum 
  • Per sample
 
Small RNA Sequencing sum 
  • Per sample
 
Gene deletion using Cas9/CRISPR in mice 
  • Per gRNA
 
CRISPR in vitro transcription for RNA Injections 
  • Per gRNA
 
Homologous Donor Design and Construction 
  • Per sample
 
Pronuclear Injection (100 embryos/4 surrogates) and Embryo transfer   
Genotyping Design and Service 
  • Per sample
 
Bioinformatics and BioMark Service 
  • Per hour
 
Cage/per day 
  • Price will vary for external/corporate users based off of your project needs.
 

Service list


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Core Services (13)