Single Cell Molecular Expression

Overview of Services


The Single Cell Molecular Expression Core (SCME) provides the following state of the art services:

 

  • Automated single cell capture followed by cDNA preparation using the Fluidigm C1 Single Cell Auto-Prep system
  • High throughput quantitative PCR assays and targeted template enrichment using the Fluidigm BioMark HD system
  • Manipulation of a gene of interest to achieve over-expression, reduced-expression, complete inactivation, or replacement with another gene using the latest genome editing technologies including ZFNs, TALENs and Cas9-CRISPR in cultured cells.
  • Assistance in the design, execution and analysis of next-generation deep sequencing and genome editing experiments.

 

iLab license provided by the NV INBRE Administrative Supplement, a grant from the NIH National Institute of General Medical Sciences (GM103440-14A1S1).

Leadership

Wei Yan | Director

Chong Tang | Co-Director

Location and hours of operation

Hours Location

Monday - Friday         

9am - 5pm 

Center Molecular Medicine Rm 202 & 204

Reno, NV, 89557 

Links and Resources

Contacts

Name Role Phone Email Location
Wei Yan
Director
 
775-784-7765
 
wyan@medicine.nevada.edu
 
CMM Room L207
 
Huili Zheng
Lab Manager
 
775-682-8873
 
hzheng@medicine.nevada.edu
 
CMM Room L203D
 

Service list


Search available services: View: by category alphabetically
Core Services (13)